Wednesday 23 February 2011

DRUG DISCOVERY & HEALTH

A healing rose  
A rose by any other name will be as sweet, wrote Shakespeare. Ever sensuous and sweet, the splendour of rose is everywhere, enchanting romance and love. But other than romancing, a rose can also speak serious matters. There is medicine in a rose.
  
   ROSES have been grown as garden plants and cut flowers for decor, fragrance and flavouring. Now there is no less than 7,500 varieties of rose. Not only scenting a garden and beautifying homes, history also shows roses have been used in medicine, food, perfume, and health. They have essential oils for perfumes widely used in cosmetic. The scent of a rose can be in many forms - rose water, rose petals, rose buds, and rose oil.

Knowing there are many uses of roses, researchers Roziana Mohamed Hanaphi and Dalina Samsudin worked to find another use of a rose - an antibacterial agent. Rose extracts were prepared from fresh roses and rose residue using solvents of different polarity.

Influenced by how our forebears used plants to heal diseases, Roziana and Dalina studied that botanical extracts have long been used to treat disease. Plants are a rich source of valuable compounds and have been a source of primary health care in many developing countries. These compounds are the active principle of many drugs. Thus screening of such plant extracts for antimicrobial activity has always been of great interest to scientists to look for new sources for food additives, cosmetics and drugs.

Thus likewise, Roziana and Dalina saw that roses have potential against microbial activities. They attempted to prove that the ethnobotanical use of rose petals can be a cure for diarrhea and enlarged tonsils, commonly caused by E.coli. In their experiment, an extraction process using several solvents was conducted, testing the extracts for antibacterial activity on Escherichia coli - the gram negative bacteria, through Kirby-Bauer disc diffusion method.

The petal tissues of flowers may possess antibacterial activity as their natural protection system for reproduction and further perpetuation through seed formation. So some rose varieties have been studied for different activity potential at genotypic level. They were found to be active against the gram-positive and gram-negative bacteria with differences in activity profiles.

Usually, the different polarity compounds are extracted from roses but in this study, it was extracted from the roses residue. Extracted roses usually contain linalool, phenylethyl alcohol, citronelol, nerol and geraniol. While the main compounds of rose extracted by solvent extraction are isopropyl myristate, rhondinol, 1-nonadecene and heneicosane. These compounds can be detected by gas chromatography with a spectrometry apparatus and generally, they have different polarity where they will be separated into similar groups of polarity solvent.

As the objective of the solvent extraction method is to separate the organic mixture into a similar groups of compounds, theoretically, the extracted polar compound has the tendency to be with the polar solvent. Hence the non polar compound will be rather chosen to be the non polar solvent.

To find antibacterial values, Roziana and Dalina employed the Kirby Bauer disk diffusion susceptibility test. It was to determine the susceptibility or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds. They screened the extracts for antibacterial activity where a paper disc was soaked with the rose extracts then laid on the top of an inoculated agar plate.

During incubation, each chemotherapeutic agent diffuses out from the disc in all directions. Agents with lower molecular weights diffuse faster than those with higher molecular weights. Clear areas, called zones of inhibition, appear on the agar around discs where the agents inhibit the microorganism. An agent of large molecular size might be a powerful inhibitor even though it might diffuse only a small distance and produce a small zone of inhibition.

Good news folks, Roziana and Dalina found that the fresh rose ethyl acetate extract showed the most promising result tfrom the other four extracts. It showed a 39 mm zone of inhibition on Mueller Hinton agar, the largest diameter zone compared to the other extracts, indicating that the Gram-negative bacteria of E.coli were highly susceptible to the extract. This means the rose extract was potential to kill or retard the growth of E.coli. They concluded that the intermediate polar compounds in roses either fresh or residues, are giving great potential as antibacterial agent in order to inhibit the E.coli.
A rose not just romances, it heals.

Information contacts:
Roziana Mohamed Hanaphi
Dalina Samsudin
Faculty of Applied Sciences
UiTM Perlis
roziana@perlis.uitm.edu.my
dalina@perlis.uitm.edu.my 



Steaming hot meehun soup 

  IT is great to eat steaming hot noodles, sipping hot meehun soup and drinking teh tarik at stalls and mamak shops. Fast and simple, the hot noodle soup is served in plastic bowls and eaten with plastic forks and spoons. Then we buy hot noodles for meals at home packed in polystyrene containers. But Hazlina Husin and her professor, Ku Halim Ku Hamid, saw danger in steaming meehun soup and noodles in these containers. 
Plastic products are used by all every day. Convenient, cheap and handy, but putting hot food in plastic containers is dangerous said scientists. They explain that there are a few types of plastic food containers, having certain properties developed during their manufacturing, but even though plastic food containers are made to follow their industrial specifications, these requirements are sometimes brushed off by manufacturers and consumers.

The lack of awareness by users about plastics organic chemistry poses more danger. Many do not know that plastic products have constraints that limit their uses, such as limited hardness, density, ability to resist heat, oxidation, organic solvents and ionizing radiation. The possibility of chemical compounds, leachate, diffusing into the food in the plastic food containers is high. This leachate is toxic and carcinogenic.

Leachate is a term used in the environment for effluent produced by a new or used material(s). Leachate from plastics food containers dumped in dumpsites will also diffuse into the surroundings and affect soil and rivers.
Worse, food-safety problems tend to rise when plastics are used for a purpose other than what they are designed for, such as polystyrene and microwave-safe food containers used to pack hot foods. Due to organic chemistry limitation, chemical compounds in polystyrene could diffuse into the food or drink in it. It is studied that leachate diffusion will be worse if the food is hot and placed longer in them.

Hazlina and her professor evaluated the possibility of a leachate flow from polystyrene and microwave-safe food containers to food. They conducted an experiment to study this diffusion using two types of common food containers, polystyrene and microwave-safe, which were cut into small pieces. A stainless steel variable-speed heating container was used and our favourite chicken soup and meehun soup, were the contact medium.
The stainless container then was filled with 200 mL of soup. Gradually, the cut plastic food container was added into it. The temperature of the container was set at 30, 50, 60, 80 and 90oC per testing batch. Food samples were then taken from the sampling container after 15 minutes. After that, the test was continued for variable storage time: 10, 15, 20, 25 and 30 minutes per testing batch, then analyzed by GCMS.

The GCMS analysis identified six extractives: Dodecamethylcyclohexasiloxane, tetradecamethyl cycloheptasiloxane,1,2-benzenedicarboxylic acid diethyl ester, hexadecanoic acid methyl ester, 1,2-benzenedicarboxylic acid dibutyl ester and pctadecenoic acid methyl ester. These extractives were the components of the leachate that diffused from the cut food containers.

Hazlina found that the highest peak identified in the extractions was the 1,2-benzenedicarboxylic acid diethyl ester, also known as phthalates esters. Phthalates esters have attracted great public attention because of their carcinogenic and estrogenic properties, which are unfortunately used as one of the plasticizers in plastics products.

Another series of test on ionization count of each chemical compound to evaluate the possibility of leachate diffusing into food was conducted. She found that more leachate was found in the food samples when the polystyrene food container was used. Leachate diffused from a polystyrene food container at a temperature of more than 30oC. Microwave-safe- food container was found to be safer when it was shown that the leachate diffused only at a temperature higher than 50oC. Likewise, it showed that the higher the temperature of food, the more the leachate diffused. She found this trend in both types of food containers.

Polystyrene is made from one of the most important and global industrial chemicals commonly used for food-contact packaging materials. Unfortunately, human exposure to styrene happens by the diffusion of these food packaging materials into food. It is estimated that human daily styrene exposure is in the range of 0.2 to 1.2 µg/person.

Leaches of styrene, an endocrine disruptor mimicking the female hormone estrogen, has potential to cause reproductive and developmental problems. Styrene diffuses significantly from the polystyrene containers into food when the foods are heated in them. In contrast, microwave-safe containers have little leaches and found to be safer even at high temperatures. These containers are made of polypropylene which are considered ‘safer’.
Their analysis also showed that the diffusion of leachate into the chicken soup and meehun soup in the experiment was also related to how long the food was placed in it. In this study, chicken soup was used as the contact medium, which was stored at 50oC in a polystyrene. Within 5 minutes, the TIC of 1,2-benzenedicarboxylic acid diethyl ester increased from 0 to about 500. Hazlina commented that the diffusion rate for the leachate here was high at this temperature. It was also found that the leachate diffused after 10 minutes of food storage time. So, if hot soup is placed in polystyrene for more than 10 minutes, we can say that there is a high possibility that leachate will diffuse into the soup.

Here Hazlina and her professor managed to show that the public should be aware of the danger of eating or putting hot foods in this type of containers. They also suggested that a comprehensive study on the stability of chemical bonding in the food containers and leachate flow into food be done on biodegradable plastic food containers. There is also a need for a database of leachate monitoring to measure the safety level of leachate-content in food.

Or after all it is good to eat our steaming meehun soup from Tok Mak‘s old bowls.

Information contacts:
Hazlina Husin
Ku Halim Ku Hamid
Faculty of Chemical Engineering
UiTM Shah Alam
hazlina858@salam.uitm.edu.my

BRAIN & NEUROSCIENCE

Halting the ß-amyloid 

    HE asks the same question every five minutes, complains he hasn’t eaten although he just eats. Every day, while doing things for him I wipe my tears secretly. It kills me to see daddy staring at me, lost and forlorn, not knowing who I am, the apple of his eyes - wrote a reader to a newspaper editor. The writer’s father is suffering from Alzheimer’s disease.

Alzheimer’s disease (AD) is a neurodegenerative disease, clinically characterised by a progressive decline in memory and cognitive function. The patient’s short-term memory gets shorter each day and they will forget almost immediately what they have asked or what they just did. They will pose simple questions like if they have eaten although they eat only minutes ago. Pitifully, AD not only affects the patient, it also does the caregivers. It takes a caregiver an extra measure of courage and patience to handle them.

AD inevitably leads to death due to various complications. In the western countries, AD is the fourth leading cause of death after heart diseases, cancer and stroke. Sadly, it is estimated 17 to 25 million of the world’s population are currently affected by AD, and the number is rising as we live longer. At the age of 65 years, an estimated 3% of the human population are affected by the disease and by the age of 85 years, the prevalence reaches up to 50%. The United Nations (UN) population projections estimate the number of people older than 80 years will be a staggering 370 million by year 2050, thus more than 100 million people aged over 85 years old will suffer from AD in the next 40 years.

Likewise in Malaysia, the prevalence estimate for AD in 2006 was 63,000 out of 23.4 million total population and the estimate for total societal cost of dementia which includes direct costs and informal care may rise to US$511 million. The number of sufferers is projected to 126,800 and 453,900 in 2020 and 2050 respectively.

Despite various efforts aimed at elucidating the aetiology of AD, there is still no effective treatment available to halt its progress. Approved drugs to treat AD, cholinesterase inhibitors and memantine, only help to treat the symptoms related to the disease. These drugs do not address the fundamental cause of the disease. However our scientists see that if we could develop drugs to treat its root, there will be a ray of hope to alleviate this frightening disease.

Abu Bakar Abdul Majeed hypothesized that to hit its roots, we need to study the ß-amyloid (Aß) plaque in the brain of AD patients. Aß is formed by an alternative cleavage of the amyloid precursor protein (APP) by the ß-site of APP cleaving enzyme (BACE1, also known as ß-secretase). Though Aß occurs in all individuals, in AD patients, it becomes screwed up hence disturbs their neuronal transmission. Hence, in this sense, to eliminate Aß, we need to give them drug that inhibits BACE1. This may help alleviate or prevent AD.

Hence, Abu Bakar, helped by his team at the Brain Research Laboratory, Faculty of Pharmacy, is currently studying to halt the ß-amyloid production in a diseased brain. To do so, they attempt to inhibit BACE1 that will reduce or virtually halt the ß-amyloid production in the AD patients’ brain. Supported by UiTM and various research grants from MOSTI, the group has tested over 300 extracts obtained from Malaysian endophytes for BACE1 inhibitory activity.

An endophyte is a microorganism that lives within a plant for at least a part of its life without causing apparent disease to its host. It is often a bacterium or fungus that produces metabolites which are not harmful to its host and could be useful to mankind. A large number of endophytes and its metabolites have still not been characterized to date and they are currently viewed as an outstanding source of bioactive natural products.

In this team’s study, out of the 385 endophytic extracts tested for BACE1 inhibitory extract, 14% showed inhibitory activity above 90%. The team has been working closely in collaboration with the University of Canterbury at Christchurch in New Zealand to determine and elucidate the structure of pure compounds found in the potent endophytic extracts responsible for the inhibitory activity of BACE1. In particular, the group has found one unknown compound coded as HAB16FC79 which has a very effective IC50 value of 0.2 μM. HAB16FC79 has a small enough molecular weight capable to cross the blood-brain barrier and work is still in progress elucidating its structure and other neuroprotective properties.

Information contacts:
Abu Bakar Abdul Majeed1
Richard Muhammad Johari James2
Jean-Frédéric Faizal Weber2
Kalavathy Ramasamy2
Vasudevan Mani2
Nur Suraya Adina Suratman3
Lim Siong Meng3
Nurul Aqmal Mohd Hazalin3
Azzeme Harun3
Nurhuda Musa3
John Blunt3
Tony Cole3
Murray Munro3
Lin Sun3
 
1Research Management Institute
UiTM Shah Alam 
2Faculty of Pharmacy
UiTM Puncak Alam 
3University of Canterbury
New Zealand
abubakar@salam.uitm.edu.my 



Making up the brain 

    MIND fleets. Brain pulses. The merging of Mind and Brain (or brain and mind?) makes up their mystique, fostering our curiosity in them.

The Quran, the Bible and other great teachings encourage Man to discover the mysteries of God, thus indeed it is - the 2000-year old Shroud of Turin examination shines the mystiques and the power of human inquisitiveness, woven in the minds and brains of scientists.

The examination resonates the Verses of Al-Baqarah, the conversation between God and His Angels at the time of human creation about the difference between having wisdom and not having one.

The Verse says “Behold, thy Lord says to the angels: ‘I will create a voce-gerent on earth’. The angel says: Wilt Thou place therein one who will make mischief herein and shed blood? Whilst we celebrate thy praises and glorify Thy holy (name)?’ God replied: ‘I know what ye know not.’ And He taught Adam the nature of all things; then He placed them before the angels and said: Tell Me the nature of these if ye are right’. They said: ‘glory to Thee, of knowledge. We have none save what Thou hast taught us: in truth it is Thou who are perfect in knowledge and wisdom’. God said: ‘O Adam! Tell them their natures’. When Adam had told them, God said: Did I not tell you that I know the secrets of heaven and earth, and I know what ye reveal and what ye conceal’?”

In the 33 A.D. Shroud of Turin, in 2009 A.D, the brains in nine fields of knowledge: theology, textile, history, DNA, carbon dating, flora and fauna, photography, 3D imaging, and lights were brought together. The scientists gathered at the Vatican, permitted to study the holy shroud in five days. They examined the linen for 122 hours, day and night, yet the mind and the brains of theology and science, still failed to demystify the Shroud of Turin if it was indeed wrapping the body of Jesus. It could only determine that the man whose body wrapped in the shroud was scourged violently, crucified cruelly and stabbed in the rib severely. The team could even bring the man’s face almost alive in a 3D dimension, but failed to determine if the man was really Jesus. Is there a Being beyond us, far beyond the brain, the mind and the science?

However, research, such as the research of the Shroud of Turin, and other form of research, seems to point that hitherto the unbridgeable gap between the psychical and the mental world of brain, mind and science is slowly narrowing.

The brain is a grey matter, carrying its own mystic and enigma, establishing the very being of a human being. Scientists call it the most mysterious organ of the human body. Thus much has been argued about the brain. However, as though it is not enough, out of this mystery, arises an even more mystifying entity - the mind. They have given rise to heated debates on the ultimate question: is mind and brain one, or are they distinctively separate? The mind is elusive, fleeting every second, shown in thoughts, languages, and perceptions, so does directing feelings, emotions and recalls.

Neuro-physiologists describe the brain as just a 1.4 kg grey matter containing approximately 100 billion nerve cells. The nerve cells are also called neurons. The neurons communicate with one another, busily sending electrical signals along the neuronal body of axons and dendrites. Further, trillions of nerve junctions or synapses link these neurons together. Chemicals in the synapses or neurotransmitters, help bridge the neurons. Does this anatomy follow the order of the mind to make up a mind? Undoubtedly, the brain with its mind or faculty of reason does. Thus man rests well above the rest of God’s creatures.

Animal, too, think and feel. Thus they do have brains. Do they not have mind? A mother monkey will hug her dead baby for days before she let it go, thus hunters are warned not to shoot mother monkeys.

Early neuroscientist, Andreas Vesalius (1514-1564 A.D.), a native of Brussels, in his landmark publication On the Workings of the Human Body, indicated that the cavities of the human brain were not different from those of other mammals. However, they do not have similar reasoning powers. Thus Adam’s ability to learn, understand and remember positions him as a superior being, despite being the latest addition to God’s creations.

Vesalius further wrote: ‘all our contemporaries, so far as I can understand them, deny to apes, dogs, horses, sheep, cattle, and other animals the main powers of the Reigning Soul – not to speak of other powers – and attribute to a man alone the faculty of reasoning: and ascribe this faculty in equal degree to all men. And yet we clearly see in dissecting that men do not excel those animals by possessing any special cavity in the brain. Not only is the number of ventricles the same, but also all other things in the brain are similar, except only in size and in complete consonance of the parts for virtue.”

Despite this anatomical finding, in many ways the brain power of animals is rather limited. But man may, if he wishes, make use of his brain to the maximum capacity, performing important functions, maintaining proper balance or homeostasis of the body. A man’s brain, too, if he wishes, will follow his mind. If the mind decides to kill, the brain will follow, moving the body towards the killing. If the mind decides to be kind, the brain will steer the body towards cooking and bringing the food to the poor, for example. The mind needs controlling, thus there emerge education, teachers, and wisdom. Prophets and religions revealed. Does the mind control the world? If only the good mind does, the man wrapped in the Shroud of Turin would not be whipped to death; and if only the bad do, there will not be civilization.

However, the mind will not work if not for more than 50 different neurotransmitters of acetylcholine, noradneraline, dopamine and serotonin in the brain. Nor does the mind work without that large surface of the brain, despite its small size. The surface is convoluted to enhance the size of effective area, necessary for efficient handling of a huge amount of information received by the brain every second, either from inside or outside the body.

Each site of the brain surface constantly receives and processes specific information. Thus the sites for visual and auditory input are separated despite we see and hear simultaneously. We are able to do both as both sites communicate extensively via connecting neurons. The brain’s ability to process information is unsurpassed by any other machines, not even by the best computers of the day. In fact, despite the success of computer builders to simulate brain-processing strategies as the simultaneous parallels and distribute processing, computers are still far to supersede the brain’s efficiency.

The computer may be faster, but the brain is much more insightful and prudent. Thus it is not surprising to find that the brain can efficiently perform numerous complex tasks like thinking, memorizing, counting, listening, speaking, planning, making decisions and dealing with emotions. A computer will not be able to do all this in one system. Placing brain to computers, by and large, brains are agile, computers rudimentary.

The brain is the center of control of most of the internal workings of the human body. The lower region of the brain, the medulla oblongata, controls the beating of the heart, as well as the rhythmic movement of respirations. The brain triggers hormonal release thus controlling our growth, metabolism, sexual maturity and mood.

The ability of the brain to control the functioning of the various organs and systems of the body is made possible via a precise feedback mechanism. As a consequence of being the nerve center of the body, injury to the brain will result in serious handicap to the individual. Consequently, brain non-function is akin to death – brain death.
Thus God iterates the marvels of the brain in His word aql, the evolvement of the mind. The root word aql does not occur in the noun form at all in the Quran but only in the verb form, suggesting it is a dynamic application.
In the verses of the Quran, the derivatives of the word aql are used to represent activities that are performed, or need to be performed, or activities not performed despite their indispensability. Also in the Quran, activities pertaining to the mind are always referred to in a positive sense. There is no suggestion whatsoever that using the mind would lead to undesirable consequences.

The capability of the brain and mind is almost limitless. Man must not let His command go unheeded - that we have to strive, discover, and move on.


Information contacts:
Abu Bakar Abdul Majeed
Megawati Omar
Research Management Institute
UiTM Shah Alam
abubakar@salam.uitm.edu.my
megawati@salam.uitm.edu.my

FRONTIER MATERIALS & INDUSTRY APPLICATION

Charging our handphones 


 CHARGING our handphones increases our electricity bill. Sure - mom never fails to remind everyone in the house to turn off the light in bedrooms, shut down computers, and switch off the aircons. Reminders to save electricity are everywhere - homes, offices, schools, factories. Just how much a month are we paying for the power we use and ‘pollute’?

Well, paying high bills is not all due to our electricity use. One of them is due to ‘pollution’ to the electrical supply system. Student Muhamad Taufiq Ramly of the Faculty of Electrical Engineering has re-constructed
a method, which was invented by lecturer Rahimi Baharom, that may help us reduce our electricity bill. Muhammad Taufiq has created a method to reduce the ‘pollution’ to electricity supply network which may have caused us the extra bill.

Conventional battery chargers of all portable devices and mass charging in factories usually introduce pollution within the power supply network. This pollution is in the form of impurities, scientifically called harmonic. Hence this means when we charge our handphones we are going to load the power system with harmonics, thus polluting the supply system network. As millions are charging handphones, the richer the harmonics be, the dirtier the supply gets. Dirty supply system incurs problems like power breakdown, higher consumption, greater loses of supply, overheating, extra energy use, lower performance, and finally higher electricity bill. Everyone will be affected.

But Muhammad Taufiq’s invention, an active power filter, will stop the ‘dirt’, assisting our charging not to pollute the power supply. He creates a measure to control the shape of the input waves, putting them back into its sinusoidal form, thus producing them smooth and clean of harmonics. As the shape of the polluted wave is changed into smoother ones the gadgets will introduce minimal harmonics.

The feature of Taufiq’s circuit is that it contains a single switch to provide an active power filter function. Most battery chargers we are using now employ filters. The active power filter here is used to mitigate the distorted current by injecting equal but opposite current to shape the pulsating of the supply current to a sinusoidal form. This is to ensure that the pulsating is in rhythm with the supply voltage. In this work, the single switch active power filter was used to reduce switching stress, losses and finally the cost. A boost converter concept was used as it would produce an output voltage greater than the input voltage. This condition makes the current flow to the inductor and thus fully charged. The energy stored in the inductor then can be used to compensate. When the switch is turned off, the diode is forward biased. The current would now flow through the inductor, diode, capacitor and load in a battery.

However, the inductor voltage reverses its polarity in an attempt to maintain its constant current. Due to the energy remains in the inductor, it is used to charge the capacitor and hence transfers the stored energy. As a result, output voltage is higher than the input voltage. Thanks to Taufiq and Rahimi, less worry when charging our handphones, we look forward to paying lower bill.


Information contacts:
Muhamad Taufiq Ramly
Rahimi Baharom
Faculty of Electrical Engineering
UiTM Shah Alam
taufiq.ramly@gmail.com
mie-2344@yahoo.com.my 



Scaling the dye away

  
   GIVE a poor man a fish, he eats for a day, teach him to fish he eats for a lifetime. Give a researcher a fish he will trade the scales. So folks, when cleaning the fish, do not throw the scales. Fish scales, seemingly useless they look, awfully smelly they be, they are valuable to scientist Hanafiah Zainal Abidin.

Taiwanese, Japanese and Chinese companies have been trading fish scales by millions of dollars. Traded for collagen and other materials for makeup products, it is about time Malaysia needs to save her fish scales. But in this case, Hanafiah is examining the use of fish scales to clean wastewater of batik factories.

Batik manufacturing is one of the largest industrial users of process water in especially extensive batik processing. Batik needs vibrant colours thus dyeing is its lifeline. To get sharp colours, manufacturers use concentrated dye, thus the dirtier the effluent is. Alas its drawbacks are that it will produce effluent filled with residual of dyes and chemical. The residual is with color, high pH, high Chemical Oxygen Demand (COD), and low biodegradability.

Thus in this case, cleaning the effluent needs a proper treatment before releasing it into the environment. In this study, coagulation-flocculation method for wastewater treatment will be applied. Polymeric coagulant produced from fish scale will be used in the process. This coagulant is tested for real wastewater from industry via jar tests.
The purpose of this research is to study the feasibility of fish scale as a source of cost effective coagulant for textile wastewater treatment. It is also to determine the optimize operating condition such as dosage and pH in textile wastewater treatment using fish scale.

With cheap raw material and polymeric content in fish scale, it is expected that economic treatment of textile wastewater could be established and biodegradable coagulant can be produced. Furthermore, production of coagulant from fish scale would enhance fishery activities.

The effluent from textile wet processing requires several treatment. Conventional activated sludge is able to remove large fractions of COD. Dye removal is most often achieved by sorption processes, rather than by biodegradation. For effluents with COD over 5000mg/l, anaerobic treatment becomes more significant. A combined anaerobic and aerobic treatment can also be effective to remove azodyes. However, this system removes dyes poorly; it is ineffective to decolorise textile effluent.

Removal of dyes is possible by activated carbon treatment. But it is expensive and the success depends on the type of dye and complete color removal is rarely achieved. Ozone is one of the strongest oxidizing agents readily available. It is used to reduce color, eliminate organic waste, reduce odour and reduce total organic in water. Ozone is one of the strongest commercially available oxidizers, making it popular for primary disinfection of potable water as well as for color and organic removal in wastewater applications. But, ozone cannot completely mineralize the organic dye to carbon dioxide and water even at high doses usage. This is due to the decolorisation rate decreasing with increasing initial dye color.

Up to date there is no economic and effective method to treat textile wastewater. Color from textile wastewater is hard to remove due to resistance of dyes to biological degradation where they are not readily degraded under the aerobic conditions prevailing in biological treatment plant. The color of textile effluent is unacceptable under Malaysian Environmental Regulation, besides the other parameter such as Chemical Oxygen Demand, Biological Oxygen Demand, and total iron.

Regulations for effluent and air emissions, however, are becoming increasingly stringent and more rigorously enforced, which prompt the industry to develop alternative methods for the management of process wastes. Due to these elements, balancing the environmental protection, economic viability and sustainable development is the biggest challenge in the textile industry. To keep the economic growing, a new way of treatment is vital to preserve the environment. To stay friendly to the environment, and not to create the wrath of nature, textile effluents need efficient treatment system.

One of the new treatment systems is coagulation. This method has been used to remove textile colors. Researchers claim that coagulation method using inorganic coagulants with an addition of polymer flocculant can remove colour almost 100%. Hence, as fish scale is found to contain adsorption ability, therefore, Hanafiah, in his work, will use the fish scale as coagulant. Up to date, there is no serious effort to use fish scales as a coagulant for wastewater treatment in Malaysia. Fish scales are thrown away as waste or used as fertilizers.

Thus the large amount of fish scale generated allows a good supply of biomaterial. The protein present in organic fraction in fish scale seem to be the major factor of adsorption ability. Other biomaterial in scales, such as chitosan, had proven its ability to remove dye where the charge neutralization in main mechanism for dye coagulation with chitosan is at acidic pH.

In this work, fish scales will be dried and ground before being added as coagulant in a powder form. An experimental study will be conducted on a local textile wastewater to assess the removal efficiency of the coagulation process. As we walk elegantly in our silk baju kurung, think of the fish scale.


Information contact:
Hanafiah Zainal Abidin
Faculty of Chemical Engineering
UiTM Shah Alam
hanafiah299@salam.uitm.edu.my 



Slow down 

   
    AS automobiles industry expands, much has been debated about unnecessary casualties due to road accidents. At present the rate of road accident deaths in Malaysia hovers around 24 deaths per 100 000 people. Much deliberation and thoughts have taken place on how to reduce the rate of accidents, hence, Muhammad Akram Adnan and Mohd Jamaludin Md Noor are studying to reduce the speed of cars as speed and road accidents casualties have a strong relationship.

They are to develop a geometric standard for effective and ‘friendly’ hump speed reducer that considers human factor. Humans are sometimes good in judging the impact of height but otherwise in the impact of speed. Worse, many ignore road safety and do not care about others even when driving within a school area. Other impacts of high speed on the road are air pollution, noise level and traffic safety.

Another nature of road accidents in Malaysia is that the number will rise in festival seasons thus attention is always focused on deaths on the road during the three festivals: Hari Raya, Chinese New year and Deepavali. But in actual fact deaths and injuries occur all year round which include pedestrians. Investigation of road accidents show that when pedestrians are hit by cars at 30km/hr, 5% of them will be killed, most injuries are slight and 30% suffer no injury , but those hit by cars at 50 km/hr, 45% will be killed and many seriously injured, and at 65 km/hr 85% killed.

In this case, according to Muhammad Akram Adnan and Mohd Jamaludin Md Noor, the speed reducer works by transferring an upward force to a vehicle, as it traverses. The force translates it into vertical displacement and acceleration. The displacement induces a front to back pitching motion to the vehicle occupants. As speed increases the amplitude, i.e. the vertical displacement, and pitching also increase. The magnitudes of displacement and acceleration are the measure for the uncomfortable sensation.

For vehicle speed of less than 70 km/h this speed reducer is able to reduce speed less than the threshold value of 40 km/h, offer acceptable level of discomfort for vehicle occupants, avoid contact with vehicle base (thus no vehicle damage), and maximise overall road safety for road users.


Information contacts:
Muhammad Akram Adnan
Mohd Jamaludin Md Noor
Faculty of Civil Engineering
UiTM Shah Alam
akramuitm@yahoo.com
mohdjamaludin@salam.uitm.edu.my

HUMANITIES & QUALITY OF LIFE

Gender construction in children’s literature

  

    RAMESH Nair of the Academy of Language Studies, UiTM Shah Alam, proposed that language should be accorded greater attention in the evaluation of gender construction in children’s literature or in any other literary texts produced for children.

He made such suggestion after he found that children’s literature has served as a powerful medium through which children construct messages about their roles in society, and gender identity.

Although possessing mental schemas about gender differences is helpful when children organize their ideas of the world around them, problems occur when children are exposed to a constant barrage of uncompromising, gender-schematic sources that lead to stereotyping, which in turn represses the full development of the child.
Given the powerful influence of children’s literature, it comes as no surprise then that children’s literature has been widely researched for evidence of gender biasness and stereotyping over several decades. However, such studies have focused primarily on texts written and published in the West.

The renewed emphasis on the importance of mastering the English language in Malaysia has led to a significant rise in the number of children’s books that are being locally published. Besides the popular children’s stories from the West that have long been available at leading bookstores in Malaysia, local publishers have also begun introducing more Malaysian children’s stories in the English language. It is imperative that these local publications be measured for quality as they are an important tool for the transmission of knowledge. Considering the fact that young children are the consumers of these texts, the measurement of ‘quality children’s books’ must include a close scrutiny of gender construction.

Ramesh also explored the construction of gender in a selection of Malaysian children’s literature texts in the English language. The aim was to examine the subtle gender-based messages that these texts inherently contain.
While still relying on the content analytic method of liberal feminist researchers of the past, this study aimed to move beyond looking at just surface level features. Instead, the way in which the various characters were constructed linguistically and through visual language was also examined. It was for this reason that Critical Discourse Analysis (CDA) was adopted as an approach to reading gender construction in Malaysian children’s literature. CDA is an approach that looks at how power imbalances are played out through choices made in language use and other semiotic modes. Four methods of analysis were relied upon - content analysis, lexical analysis, transitivity analysis and visual analysis.

The findings of the content analysis revealed significant imbalances in the distribution of female and male social actors, both in the roles that they played and their appearances in the accompanying illustrations. In both cases, males outnumbered females. Practices of stereotyping were found in the distribution of the characters in the various settings. While the home setting appeared established as a feminised space, the workplace and outdoor settings were dominated by males. The content analysis also pointed towards stereotyping practices in the way female and male characters were ascribed behavioural traits and status in society. The association between gender and behavioural trait appeared to firmly establish power in the hands of the male characters. In the next stage of analysis that focused on both written and visual language, strong nuances of sexism were identified in apparently neutral texts, revealing a weaker construction of females. The analysis of lexical units and clauses revealed deeply embedded linguistic structures that positioned males as predominantly more powerful than the female characters. The visual analysis focused on the roles the female and male characters took on in the illustrations and it was again found that male characters were accorded the more important roles of active doer while females took on the role of passive observer.

Ramesh concluded that an analysis of language, grounded in a CDA approach, offers researchers and decision-makers in the selection of texts for children greater insights into the way gender is subtly constructed.


Information Contact:
Ramesh Nair
Academy of Language Studies
UiTM Shah Alam
ramesh@salam.uitm.edu.my 



Discolouring wastewater 

  
    CAPTIVATING colours, fostering the allure of batik, is now adorning the silhouette of supermodels in Milan, Paris and New York. They strut in tones and tints of our batik.

However effluent flushed by batik manufacturers is filled with dyes and chemicals. Habsah Alwi and team are working to clean batik effluent using the humble jering (Pithecellobium Jiringa). We seem to have this love and hate relationship with jering, having it as our delicious delicacy and salad but abhoring its pungent smell. Alas, now we are to love it more, it will help us clean our dirty water.

Habsah sees that Jering has the knack to reduce the colors of dye in the wastewater of batik factories. Traditionally jering is a type of vegetable, where the young seeds normally eaten fresh or raw for healthy living and medicinal values, especially believed to prevent diabetes and high blood pressure. When mature, the seeds are cooked. The young leaves of its tree, dark purple in colour, is also eaten raw. Unfortunately the new generations seldom eat this traditional ulam, both seeds and young leaves, in their meals because of its strong odour.

Jering , also called jengkol by the Malay community, belongs to the family of Legumnasea, a sub family of Mimosaceae. It is believed to have originated and widely distributed in Indonesia, Malaysia and South Thailand. The seed is 3-5 cm across with yellow testa when young, turns brown when mature. The strong odour from the seed has limited the consumption of this valuable herb. It is believed that alcholoid in jering is the main composition that helps to reduce sugar level and high blood pressure. However, Jering can only be eaten regularly in small amount as djenkolic acid from this seed may form crystalline in the kidney if consumed too much.

As its popularity as a dish is waning, Habsah and colleagues investigated if jering could be used for other uses. Biodegradable and containing active ingredients, it is believed that its pod and seed can be used to reduce pollutants in wastewater. This suits the textile industries. Textile manufacturers, especially local manufacturers of batik, produce a large amount of effluent. As jering is available in abundance, it could be another good means to treat this wastewater.

In the experiment, the pot and seed were cleaned and separated, crushed and sieved to get the homogenize size. Then both of them were put into the oven at temperatures ranging from 50-1000C for one day to dry. A few samples of batik wastewater were put into several beakers and the initial characteristics of the sample were recorded. The dried seed and pod powder were poured into these beakers. The reduction in colour was observed periodically and its changes were compared with the colour of the untreated water.

In the experiment, the blue wastewater turned lighter after a contact with the dried jering. Habsah explained that this might be due to the active ingredients, djencolic acid and alcholoid. Djencolic acid and alcholoid are believed able to entrap the blue pigment through their adsorption mechanisms. Furthermore, the elements of ferum and calcium also help in agglomerating the pigments and able to settle the pigments by gravity settling. The sedimentation of pigments was filtered, leaving a lighter blue wastewater (supernatant). Then it was treated by flowing it through an activated carbon to adhere to the requirements of the Department of Environment.

Our ulam jering treating wastewater? Indeed it is. Habsah thinks that further research need to be done to investigate the active compounds that help to remove colour. The research may benefit villagers and aborigines, who trade the jering, to improve their income and well-being. Habsah’s findings may also give rise to new ideas in the search for other ‘green’ resources to treat textile wastewater.
What about researching our humble ‘petai’?

Information contacts:
Habsah Alwi
Radziah Wahid
Nor Hazelah Kasmuri
Suhaiza Hanim Hanipah
Fauziah Marpani
Faculty of Chemical Engineering
UiTM Shah Alam
habsahalwi@salam.uitm.edu.my 



No more smelly drains 

 THE drain may not be big but its smell is annoying. Many ways are suggested to avoid smelly drains – do not throw food in drains, pour boiling water, throw salt, put baking soda, use distilled vinegar and many more. Master’s student Saliha Mohamad and her professor, Suhaimi Abdul Talib of the Faculty of Civil Engineering, UiTM Shah Alam, discovered that sulphur and nitrogen can help us get rid of this old age problem.


One of the ways water in drains can be smelly is due to the transformation of sulphur compounds. Anaerobic decomposition of wastewater in sewer systems that generate sulphur compounds have been identified as a major cause of problems of toxicity, foul odours and corrodibility. This happens due to the transformation of sulphur compounds in wastewater that forms hydrogen sulphide.

Also, the emission of sulphide in sewer systems corrodes the sewer structures. It makes them smelly where sometimes wastewater treatment remains ineffective. Sulphide, as well as other volatile organics compounds, are very toxic and can emit obnoxious odour even at low concentration levels (10 mg/L). It is produced when organic matters decompose in the sewer system. Worse, lack of ventilation and reaeration in the sewer system makes it smellier. Hydrogen sulphide, which smells like rotten eggs, is also flammable and very poisonous to us and animals, causing death at high concentration.

Usually, sulphide will be produced more easily and faster in warmer climates countries. Thus Malaysia, being a hot-climate country, has a serious problem in her sewer systems in the midst of anaerobic conditions of hydrogen sulphide. Not only hot climate countries, temperate climate countries also face great problems of concrete deterioration in their sewer systems. The US Environment Protection Agency (US EPA) had investigated sewer systems in 131 cities and reported that 66 of them had corroded through the emission of sulphide into the sewer atmosphere as hydrogen. Then hydrogen sulphide will diffuse into a thin liquid film on the sewer surfaces. Here, chemoautotrophic bacteria will oxidize the sulphide to sulphuric acid. The biogenic sulphuric acid will react with the cementitious material of the concrete and corrode the sewer wall, eventual causing structural failure of the sewers.

The US EPA reported in 1991 that the range of concrete deterioration rate in the country’s sewer pipes was 2.5-10 mm year in its investigation of 34 cities. Similar observations were made in Denmark and Portugal. Corrosion of sewer walls will then cause ex-filtration of wastewater into groundwater, polluting the receiving waters, making it unhealthy and smelly.

Thus Saliha and her professor theorized that the microbial transformation in wastewater during transportation can improve the performance of wastewater system. As basic kinetics of anoxic sulphide oxidation in sewer systems serves as an important tool to reduce the hydrogen sulphide, (by adding of nitrate) they discover that odour can be reduced. Secondly, as sulphide is reduced, the workers responsible for sewer systems will also be protected from its toxicity. Thus the corrosion of sewer wall also can be reduced.


Their test was conducted on wastewater from a manhole near the In-Sewer Processes Laboratory of the Faculty of Civil Engineering, UiTM Shah Alam. The wastewater was collected as a raw wastewater sample in the peak rate of wastewater flow between 8:30 am to 9:30 am. The wastewater was collected from three different places that were, the Faculty of Office Management, the Faculty of Applied Sciences and the cafeteria. The grab sampling method was used to collect the sample wastewater. To ensure that the anaerobic condition occurs naturally and similar to the condition in the sewer system, the collected samples were kept in an air tight container for 14 days before being tested in a batch test.

After the test, they found that the wastewater in sewer systems could oxidize the sulphide biologically under anoxic conditions, thus they created a process to oxidize sulphide to sulphate in a single-step process. Also, nitrate was reduced to nitrogen gas through a two-step process. Hence less odour occurred then.

The result of this study usable by our local authorities to provide guidelines to enhance municipal wastewater treatments in sewer systems. On top of that, the results may be useful for wastewater engineering companies in Malaysia to upgrade the existing sewer systems to reduce odour, prevent corrosion problems and reduce health risk from hydrogen sulphide formation.

In the same vein, this study has also contributed to establish basic kinetics and pathway of sulphide oxidation under anoxic condition in bulk water phase. However, it is recommended that investigation on the transformation rate of sulphide in biofilm to establish full anoxic sulphur oxidation be conducted. Likewise, studies on micro level should be conducted as knowledge on microrganisms activities at molecular level will enhance understanding on microbial transformations of sulphur compounds.


Information Contacts:
Saliha Mohamad
Suhaimi Abdul Talib
Faculty of Civil Engineering
UiTM Shah Alam
ecsuhaimi@salam.uitm.edu.my
sal_saliha@yahoo.com 



Marrying Mathematics and tudung saji 


  AT the Plato’s Academy in Athens, reads ‘Let no one ignorant of Mathematics enter here.’ Recall our Mathematics class at school? It did crack and tease our brain somehow.

Being one of the oldest fields of knowledge, Mathematics, introduced by the ancient Greeks, Arabs and Indians, now is assisting the tudung saji weavers in Malacca and Terengganu – deepening the art and mystique of the tudung saji.

Mathematician Daud Mohamad of the Faculty of Computer and Mathematical Sciences and his research team conducted an ethnomathematics study on tudung saji weaving.

The tudung saji is a type of food cover used by Malaysians, especially those in rural areas. They are a woven conical shape cover, from the strands of mengkuang (Pandanus family) or a type of palm plant called sal (Licuala species). The pandan strands, dipped in dyes, dried, and later softened, are woven in a specific technique called triaxial or hexagonal weave, where the strands are plaited in three directions.

Weavers use five-strand plaits to weave a tudung saji. Traditionally, five strand-weave is the most favorable to get the right proportion of shape and size. The weaving is begun by building a cone-shaped latticework of triaxial weave. The latticework, functioning as a framework, is begun by plaiting five strands to form a pentagonal opening. This is followed by interlacing another five strands at the vertices of the pentagon to form five hexagonal openings. Adding five strands each time will enlarge the structure, developing it into a conical shape. From the edge of the cone, colored strands are then interlaced upward and across in between the openings on the framework, resulting in a hexagonal tessellation, presenting an illusionary three-dimensional cubes. The illusion deepens the mystique of a tudung saji.

Interestingly though, in this study, the weavers claimed to Daud that more patterns could be accomplished if the weaving of the framework favors the right strands (where the right strands are placed on top of the left strands) and the five-pointed star at the top is turned counter-clockwise. As experienced weavers, they also claimed that a left-handed weaver will produce a different pattern of weave had he or she woven right-handedly. They concluded that a left-handed weaver weaves a different pattern from does a right-handed weaver.

However, Daud and his colleagues, engaging their Mathematical skills and logical mind, thought otherwise. They argued that the employment of either left-hand or right-hand weaving leaves no impact on the number of patterns produced. To them, the patterns created by left-hand weaving are just mirror images of the ones that of a right-hand weaving. Both patterns are just chiral to each other.

Daud and colleagues also believed that the turning of the five-pointed star, either clockwise or counter clockwise, should not make any difference in the number of patterns created. They said that it was that there are two chiralities in weaving. Thus if a cover is made with a particular chirality, then a similar one could also be produced with the opposite chirality. Hence, the weavers’ claim was deemed unacceptable.

Then, they ran an experiment. The weavers told that the weaving of the framework must begin with five strands of pandan to form a curvature at the pentagon and attain the conical shape. But the mathematicians mulled this over and wondered whether there was some significance attached to this number, and whether it would be possible to build the framework with a different number of strands. They accepted the weavers’ claim that the structure would lie flat if the weaving was started with six strands, but they were curious and wanted to know what would happen if the framework was begun with three, four or seven.

In the first trial, a conical-shaped, triaxially-woven latticework that was started with four strands was built based on the structural construction of an old Chinese hat. This structure was shown to the weavers and they were invited to reconstruct it and figure out possible ways of filling up the openings. Since they had always believed that the cone shape could only be obtained with a starting point of five strands, all of the weavers were quite amazed to see that if the cone shape could still take shape if only four strands were used instead.

After several attempts, they succeeded in forming a peak out of four strands, and proceeded to fill up the rest of the openings. However, due to its shape, which when compared to the regular 5-strand peak cover, is sharper at the apex and narrower around the edge, the weavers unanimously decided that the new structure was unsuitable to be used as a food cover.

Two of the weavers later claimed that it is possible to make a 3-strand-peak, conical tudung saji. However, the triaxial weave occurs only at the apex, and the succeeding weaving technique follows the rectangular mat weaving style, where the strands are interlaced perpendicularly to each other. In other words, this piece of work does not permit any openings and therefore requires no insertions. As a result, it allows no creation of the typical tudung saji patterns.

This style is unpopular with the weavers because it is much more difficult to make, time-consuming, and costly since it requires the use of more strands. Furthermore, only weavers who are skilled at mat-weaving patterns can weave a tudung saji of this type.

However, the mathematicians helped the weavers see a new concept in weaving and to a certain extent, change their views in weaving. Thus later, they produced a 2-peak and 3-peak tudung saji which helped them gain more sale. An innovative narrow tudung saji that one of the weavers built by interweaving 4 strands at the starting point which produced a 2-peak tudung saji, sold more. Customers seemed to appreciate a high tudung saji as it can cover tall objects like a tea set.

Isn’t it stylish to cover your tea sets? Mathematics is a challenging but an engaging discipline, training the mind and stretching imagination, in this case, the weavers’.


Information Contact:
Daud Mohamad
Faculty of Computer and Mathematical Sciences
UiTM Shah Alam
daud201@salam.uitm.edu.my 



Reviving the Iban alphabet 

   
    BROMELEY Philip of UiTM Sarawak attempted to revive the Iban alphabet. The Iban (Iban refers to people) is a plural term in itself, similar to the usage of the term Chinese) of Sarawak. It does not possess any form of writing system that can be universally used by its large community. There was however an interesting individual effort at inventing some forms of a writing system. One known writing system is that invented by Dunging anak Gunggu, acknowledged in the Encyclopaedia of Iban Studies (2001). This Iban Alphabet is not an ancient system of writing as the inventor lived well into the mid-80s. The alphabet manifests the dynamism of a modern alphabet suitable for practical inscription. It comprises syllabic writing and alphabetic writing symbols. The former consists of syllabary, representing syllables in the Iban language. The latter comprises true alphabet of consonants and individual vowels or vowel clusters.

The earliest recognition of Dunging’s Alphabet can be found in a comprehensive Iban-English Dictionary written by Anthony Richards (1981). A very meaningful recognition of Dunging’s Alphabet was made in the Encyclopaedia of Iban Studies published by Tun Jugah Foundation in 2001. His alphabet can be found under the ‘alphabet’ entry which devotes several pages to Dunging’s related work. It is very clear that Dunging’s Alphabet is the only known writing system within the Iban community which therefore strongly suggests that his alphabet could be the only alphabet ever to have come into existence within the Iban society. In this respect, it is possible therefore to acknowledge that Dunging’s Alphabet may well also be known as the Iban Alphabet. In 1947 Dunging invented 77 characters/symbols representing phonological sounds in the Iban language. His alphabet was taught to a few of his nephews while the rest of the people in his community were too illiterate to appreciate the significance of his alphabet then. Undaunted by the poor response from the surrounding community, Dunging kept revising and refining his alphabet until after almost 15 years he managed to discard some overlapping and redundant characters.

The alphabet is practical in terms of usage as it can be used to spell Iban words accurately. There are several sounds in the Iban language that cannot be distinguished distinctively in terms of spelling using the Roman alphabet; the word ‘mata’ (for eye) and ‘mata’ (for raw – fruits/food). The Iban alphabet however can distinguish between the exact sounds of the syllables to represent each sound in accurate orthography. In fact, it is very clear that Dunging’s Iban alphabet is a comprehensive system of writing that provides sufficient characters representing accurate syllabic sounds in the Iban language. The alphabet is characterised by features of modern alphabet. It comprises 59 characters whereby 16 characters symbolise consonants and the rest (43) represent vowels in the forms of vowel clusters and diphthongs. The Iban alphabet in its computer fonts is known as LaserIban which is available in the form of software for installation in computers. LaserIban fonts for Windows and Macintosh are completely cross-platform compatible, as regular Word files, in the same manner as regular English fonts. By developing the characters into computer fonts, the system of writing will become more systematic for inscriptional purposes. With a systematic set of alphabet, it should enable the users to use it more conveniently considering the fact that there are 59 characters to remember. In other words, a systematic system of writing enables its practical use with much ease.

Information contact:
Bromeley Philip
Academy of Language Studies
UiTM Sarawak
jurorbp7@lycos.com
bromeley@sarawak.uitm.edu.my